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aa 2 212  (R&D Systems)


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    Structured Review

    R&D Systems aa 2 212
    Aa 2 212, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+bcl+2+protein/Recombinant+Human+Bcl-xL+(minus+C-Terminus)+Protein%2C+CF/pmc13046825-412-21-26
    Average 94 stars, based on 5 article reviews
    aa 2 212 - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Labeling:

    Article Title: Desensitized chimeric antigen receptor T cells selectively recognize target cells with enhanced antigen expression.
    Article Snippet: .. Briefly, EBV LCLs were labeled using a CellTrace violet cell proliferation kit (C34557, Thermo Fisher Scientific, Inc.) and co-cultured with each type of T cell at a T cell:EBV LCL ratio of 5:1 for 4 h in the presence of anti-CD178 (FasL) antibody (FasL blocker; unconjugated; 10 μg/mL; 556371, BD Biosciences; Supplementary Table 2), anti-CD253 (TRAIL) antibody (TRAIL blocker; unconjugated; 10 μg/mL; 550912, BD Biosciences; Supplementary Table 2), concanamycin A (CMA; perforin-1 blocker; 1 μg/ mL; C9705-25UG, Sigma-Aldrich, St. Louis, MO, USA), or recombinant human Bcl-2 Protein (granzyme B blocker; 1 μg/mL; 827-BC, R&D Systems, Minneapolis, MN, USA). ..

    Article Title: Desensitized chimeric antigen receptor T cells selectively recognize target cells with enhanced antigen expression
    Article Snippet: .. Briefly, EBV LCLs were labeled using a CellTrace violet cell proliferation kit (C34557, Thermo Fisher Scientific, Inc.) and co-cultured with each type of T cell at a T cell:EBV LCL ratio of 5:1 for 4 h in the presence of anti-CD178 (FasL) antibody (FasL blocker; unconjugated; 10 μg/mL; 556371, BD Biosciences; Supplementary Table ), anti-CD253 (TRAIL) antibody (TRAIL blocker; unconjugated; 10 μg/mL; 550912, BD Biosciences; Supplementary Table ), concanamycin A (CMA; perforin-1 blocker; 1 μg/mL; C9705-25UG, Sigma-Aldrich, St. Louis, MO, USA), or recombinant human Bcl-2 Protein (granzyme B blocker; 1 μg/mL; 827-BC, R&D Systems, Minneapolis, MN, USA). ..

    Recombinant:

    Article Title: Desensitized chimeric antigen receptor T cells selectively recognize target cells with enhanced antigen expression.
    Article Snippet: .. Briefly, EBV LCLs were labeled using a CellTrace violet cell proliferation kit (C34557, Thermo Fisher Scientific, Inc.) and co-cultured with each type of T cell at a T cell:EBV LCL ratio of 5:1 for 4 h in the presence of anti-CD178 (FasL) antibody (FasL blocker; unconjugated; 10 μg/mL; 556371, BD Biosciences; Supplementary Table 2), anti-CD253 (TRAIL) antibody (TRAIL blocker; unconjugated; 10 μg/mL; 550912, BD Biosciences; Supplementary Table 2), concanamycin A (CMA; perforin-1 blocker; 1 μg/ mL; C9705-25UG, Sigma-Aldrich, St. Louis, MO, USA), or recombinant human Bcl-2 Protein (granzyme B blocker; 1 μg/mL; 827-BC, R&D Systems, Minneapolis, MN, USA). ..

    Article Title: Enzymatically active cathepsin D sensitizes breast carcinoma cells to TRAIL.
    Article Snippet: Cathepsin D (CD), a ubiquitously expressed lysosomal aspartic protease, is upregulated in human breast carcinoma and many other tumor types.. CD has been repeatedly reported to act as key mediator of apoptosis induced by various chemotherapeutics.. However, there is still controversy over the role of enzymatic/proteolytic versus protein-protein interaction activities of CD in apoptotic signaling.

    Article Title: Desensitized chimeric antigen receptor T cells selectively recognize target cells with enhanced antigen expression
    Article Snippet: .. Briefly, EBV LCLs were labeled using a CellTrace violet cell proliferation kit (C34557, Thermo Fisher Scientific, Inc.) and co-cultured with each type of T cell at a T cell:EBV LCL ratio of 5:1 for 4 h in the presence of anti-CD178 (FasL) antibody (FasL blocker; unconjugated; 10 μg/mL; 556371, BD Biosciences; Supplementary Table ), anti-CD253 (TRAIL) antibody (TRAIL blocker; unconjugated; 10 μg/mL; 550912, BD Biosciences; Supplementary Table ), concanamycin A (CMA; perforin-1 blocker; 1 μg/mL; C9705-25UG, Sigma-Aldrich, St. Louis, MO, USA), or recombinant human Bcl-2 Protein (granzyme B blocker; 1 μg/mL; 827-BC, R&D Systems, Minneapolis, MN, USA). ..

    Article Title: A multi-scale systems pharmacology approach uncovers the anti-cancer molecular mechanism of Ixabepilone.
    Article Snippet: It has been realized that FDA approved drugs may have more molecular targets than is commonly thought.. Thus, to find the exact drug-target interactions (DTIs) is of great significance for exploring the new molecular mechanism of drugs.. Here, we developed a multi-scale system pharmacology (MSSP) method for the large-scale prediction of DTIs.

    Incubation:

    Article Title: Enzymatically active cathepsin D sensitizes breast carcinoma cells to TRAIL.
    Article Snippet: Cathepsin D (CD), a ubiquitously expressed lysosomal aspartic protease, is upregulated in human breast carcinoma and many other tumor types.. CD has been repeatedly reported to act as key mediator of apoptosis induced by various chemotherapeutics.. However, there is still controversy over the role of enzymatic/proteolytic versus protein-protein interaction activities of CD in apoptotic signaling.



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    Spheroids show heightened apoptotic sensitivity to extracellular Mg 2+ imbalance. ( a ) Representative Western blots of p53, <t>Bcl-2,</t> Bax, caspase-3, and cleaved caspase-3 in parental (2D) and spheroid-derived (SP; 3D) HT-29 cells cultured under control, moderate low Mg 2+ (0.4 mM; low-Mg), and moderate high Mg 2+ (2.5 mM; high-Mg) conditions. We conducted a quantitative analysis of ( b ) p53, ( c ) Bcl-2, ( d ) Bax, ( e ) caspase-3, and ( f ) cleaved caspase-3. ( g ) Mitochondrial membrane potential (ΔΨm) was measured by the red-to-green JC-1 fluorescence intensity ratio; FCCP served as a depolarization control. Data are mean ± SD (n = 5). Statistical analysis was performed using one-way ANOVA with Dunnett’s post hoc test for multiple comparisons versus control groups and unpaired Student’s t -test for comparisons between parental and SP groups under the same Mg 2+ condition. The significance levels are as follows: * p < 0.05, ** p < 0.01, *** p < 0.001 versus the control parental group. †† p < 0.01, ††† p < 0.01 versus parental cells under identical Mg 2+ conditions. ### p < 0.001 versus the control SP group.
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    Spheroids show heightened apoptotic sensitivity to extracellular Mg 2+ imbalance. ( a ) Representative Western blots of p53, <t>Bcl-2,</t> Bax, caspase-3, and cleaved caspase-3 in parental (2D) and spheroid-derived (SP; 3D) HT-29 cells cultured under control, moderate low Mg 2+ (0.4 mM; low-Mg), and moderate high Mg 2+ (2.5 mM; high-Mg) conditions. We conducted a quantitative analysis of ( b ) p53, ( c ) Bcl-2, ( d ) Bax, ( e ) caspase-3, and ( f ) cleaved caspase-3. ( g ) Mitochondrial membrane potential (ΔΨm) was measured by the red-to-green JC-1 fluorescence intensity ratio; FCCP served as a depolarization control. Data are mean ± SD (n = 5). Statistical analysis was performed using one-way ANOVA with Dunnett’s post hoc test for multiple comparisons versus control groups and unpaired Student’s t -test for comparisons between parental and SP groups under the same Mg 2+ condition. The significance levels are as follows: * p < 0.05, ** p < 0.01, *** p < 0.001 versus the control parental group. †† p < 0.01, ††† p < 0.01 versus parental cells under identical Mg 2+ conditions. ### p < 0.001 versus the control SP group.
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    Spheroids show heightened apoptotic sensitivity to extracellular Mg 2+ imbalance. ( a ) Representative Western blots of p53, <t>Bcl-2,</t> Bax, caspase-3, and cleaved caspase-3 in parental (2D) and spheroid-derived (SP; 3D) HT-29 cells cultured under control, moderate low Mg 2+ (0.4 mM; low-Mg), and moderate high Mg 2+ (2.5 mM; high-Mg) conditions. We conducted a quantitative analysis of ( b ) p53, ( c ) Bcl-2, ( d ) Bax, ( e ) caspase-3, and ( f ) cleaved caspase-3. ( g ) Mitochondrial membrane potential (ΔΨm) was measured by the red-to-green JC-1 fluorescence intensity ratio; FCCP served as a depolarization control. Data are mean ± SD (n = 5). Statistical analysis was performed using one-way ANOVA with Dunnett’s post hoc test for multiple comparisons versus control groups and unpaired Student’s t -test for comparisons between parental and SP groups under the same Mg 2+ condition. The significance levels are as follows: * p < 0.05, ** p < 0.01, *** p < 0.001 versus the control parental group. †† p < 0.01, ††† p < 0.01 versus parental cells under identical Mg 2+ conditions. ### p < 0.001 versus the control SP group.
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    Downregulation of REDD1 promotes A549 and H1299 cell apoptosis under hypoxia. ( A , B ) Apoptosis of A549 and H1299 cells following the knockdown of REDD1 was detected by flow cytometric analysis of Annexin V-FITC/PI. ( C , D ) Quantitation of apoptotic cells. ( E , F ) Protein expression of Bax and <t>Bcl2</t> was determined by Western blot. n = 4. * p < 0.05; ** p < 0.01.
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    Image Search Results


    Spheroids show heightened apoptotic sensitivity to extracellular Mg 2+ imbalance. ( a ) Representative Western blots of p53, Bcl-2, Bax, caspase-3, and cleaved caspase-3 in parental (2D) and spheroid-derived (SP; 3D) HT-29 cells cultured under control, moderate low Mg 2+ (0.4 mM; low-Mg), and moderate high Mg 2+ (2.5 mM; high-Mg) conditions. We conducted a quantitative analysis of ( b ) p53, ( c ) Bcl-2, ( d ) Bax, ( e ) caspase-3, and ( f ) cleaved caspase-3. ( g ) Mitochondrial membrane potential (ΔΨm) was measured by the red-to-green JC-1 fluorescence intensity ratio; FCCP served as a depolarization control. Data are mean ± SD (n = 5). Statistical analysis was performed using one-way ANOVA with Dunnett’s post hoc test for multiple comparisons versus control groups and unpaired Student’s t -test for comparisons between parental and SP groups under the same Mg 2+ condition. The significance levels are as follows: * p < 0.05, ** p < 0.01, *** p < 0.001 versus the control parental group. †† p < 0.01, ††† p < 0.01 versus parental cells under identical Mg 2+ conditions. ### p < 0.001 versus the control SP group.

    Journal: International Journal of Molecular Sciences

    Article Title: Altered Magnesium Environments Restrict Colorectal HT-29 Spheroid Growth by Disturbing Cellular Mg 2+ Homeostasis

    doi: 10.3390/ijms27020834

    Figure Lengend Snippet: Spheroids show heightened apoptotic sensitivity to extracellular Mg 2+ imbalance. ( a ) Representative Western blots of p53, Bcl-2, Bax, caspase-3, and cleaved caspase-3 in parental (2D) and spheroid-derived (SP; 3D) HT-29 cells cultured under control, moderate low Mg 2+ (0.4 mM; low-Mg), and moderate high Mg 2+ (2.5 mM; high-Mg) conditions. We conducted a quantitative analysis of ( b ) p53, ( c ) Bcl-2, ( d ) Bax, ( e ) caspase-3, and ( f ) cleaved caspase-3. ( g ) Mitochondrial membrane potential (ΔΨm) was measured by the red-to-green JC-1 fluorescence intensity ratio; FCCP served as a depolarization control. Data are mean ± SD (n = 5). Statistical analysis was performed using one-way ANOVA with Dunnett’s post hoc test for multiple comparisons versus control groups and unpaired Student’s t -test for comparisons between parental and SP groups under the same Mg 2+ condition. The significance levels are as follows: * p < 0.05, ** p < 0.01, *** p < 0.001 versus the control parental group. †† p < 0.01, ††† p < 0.01 versus parental cells under identical Mg 2+ conditions. ### p < 0.001 versus the control SP group.

    Article Snippet: Membranes were probed with specific primary antibodies against p53 (PB9008; Bosterbio, Pleasanton, CA, USA), BCL-2 (A00040; Bosterbio), Bax (A00183; Bosterbio), caspase-3 (PB9188; Bosterbio), cleaved caspase-3 (ab32042; Abcam), pS556 ULK1 (ab203207; Abcam), p62/SQSTM1 (ab109012; Abcam), TRPM6 (0ST00108W; Thermo Fisher Scientific Inc.), TRPM7 (ab729; Abcam), MagT1 (ab244490; Abcam), CNNM4 (ab191207; Abcam), Mrs2 (ab246915; Abcam), β-actin (ab6276; Abcam), and Na + /K + -ATpase (ab76020; Abcam).

    Techniques: Western Blot, Derivative Assay, Cell Culture, Control, Membrane, Fluorescence

    A unified model of Mg 2+ -dependent fate determination in colorectal cancer spheroids. This schematic summarizes the divergent cellular and metabolic outcomes in HT-29 spheroids cultured under varying extracellular Mg 2+ concentrations. Under physiological control conditions (1.0 mM Mg 2+ ), intact TRPM6/7 heterodimers sustain Mg 2+ influx, ensuring adequate cellular and mitochondrial Mg 2+ content, maintained ΔΨm, and enhanced expression of anti-apoptotic (Bcl-2) and mitochondrial Mg 2+ -import (Mrs2) proteins. Together, these processes support spheroid integrity, growth, and survival. Both low (0.4 mM) and high (2.5 mM) extracellular Mg 2+ environments disrupt Mg 2+ homeostasis through distinct initial mechanisms that converge on a common pathogenic endpoint: Mg 2+ influx collapse, mitochondrial Mg 2+ deficiency, loss of ΔΨm, and downregulation of Bcl-2 and Mrs2. These changes trigger upregulation of pro-apoptotic (Bax) and autophagic (p62) markers, leading to increased apoptosis and autophagy, ultimately resulting in spheroid disintegration. The model underscores the dual vulnerability of colorectal cancer spheroids to Mg 2+ dysregulation and highlights the TRPM6/7–Mrs2 axis as a central node for therapeutic intervention.

    Journal: International Journal of Molecular Sciences

    Article Title: Altered Magnesium Environments Restrict Colorectal HT-29 Spheroid Growth by Disturbing Cellular Mg 2+ Homeostasis

    doi: 10.3390/ijms27020834

    Figure Lengend Snippet: A unified model of Mg 2+ -dependent fate determination in colorectal cancer spheroids. This schematic summarizes the divergent cellular and metabolic outcomes in HT-29 spheroids cultured under varying extracellular Mg 2+ concentrations. Under physiological control conditions (1.0 mM Mg 2+ ), intact TRPM6/7 heterodimers sustain Mg 2+ influx, ensuring adequate cellular and mitochondrial Mg 2+ content, maintained ΔΨm, and enhanced expression of anti-apoptotic (Bcl-2) and mitochondrial Mg 2+ -import (Mrs2) proteins. Together, these processes support spheroid integrity, growth, and survival. Both low (0.4 mM) and high (2.5 mM) extracellular Mg 2+ environments disrupt Mg 2+ homeostasis through distinct initial mechanisms that converge on a common pathogenic endpoint: Mg 2+ influx collapse, mitochondrial Mg 2+ deficiency, loss of ΔΨm, and downregulation of Bcl-2 and Mrs2. These changes trigger upregulation of pro-apoptotic (Bax) and autophagic (p62) markers, leading to increased apoptosis and autophagy, ultimately resulting in spheroid disintegration. The model underscores the dual vulnerability of colorectal cancer spheroids to Mg 2+ dysregulation and highlights the TRPM6/7–Mrs2 axis as a central node for therapeutic intervention.

    Article Snippet: Membranes were probed with specific primary antibodies against p53 (PB9008; Bosterbio, Pleasanton, CA, USA), BCL-2 (A00040; Bosterbio), Bax (A00183; Bosterbio), caspase-3 (PB9188; Bosterbio), cleaved caspase-3 (ab32042; Abcam), pS556 ULK1 (ab203207; Abcam), p62/SQSTM1 (ab109012; Abcam), TRPM6 (0ST00108W; Thermo Fisher Scientific Inc.), TRPM7 (ab729; Abcam), MagT1 (ab244490; Abcam), CNNM4 (ab191207; Abcam), Mrs2 (ab246915; Abcam), β-actin (ab6276; Abcam), and Na + /K + -ATpase (ab76020; Abcam).

    Techniques: Cell Culture, Control, Expressing

    Downregulation of REDD1 promotes A549 and H1299 cell apoptosis under hypoxia. ( A , B ) Apoptosis of A549 and H1299 cells following the knockdown of REDD1 was detected by flow cytometric analysis of Annexin V-FITC/PI. ( C , D ) Quantitation of apoptotic cells. ( E , F ) Protein expression of Bax and Bcl2 was determined by Western blot. n = 4. * p < 0.05; ** p < 0.01.

    Journal: Biomedicines

    Article Title: REDD1 Affects Proliferation, Apoptosis, Migration, and Colony Formation via p-ERK and p-JNK Signaling in Lung Adenocarcinoma Cells Under Hypoxia

    doi: 10.3390/biomedicines13122918

    Figure Lengend Snippet: Downregulation of REDD1 promotes A549 and H1299 cell apoptosis under hypoxia. ( A , B ) Apoptosis of A549 and H1299 cells following the knockdown of REDD1 was detected by flow cytometric analysis of Annexin V-FITC/PI. ( C , D ) Quantitation of apoptotic cells. ( E , F ) Protein expression of Bax and Bcl2 was determined by Western blot. n = 4. * p < 0.05; ** p < 0.01.

    Article Snippet: B-cell lymphoma-2 (Bcl2) (A00040-1) antibody was obtained from Boster (Wuhan, China). p-p44/42 MAPK (ERK1/2) (Thr202/Tyr204) (#9101), p44/42 MAPK (ERK1/2) (#4695), p-SAPK/JNK (Thr183/Tyr185) (#4668), SAPK/JNK (#9252), p-p38 MAPK (Thr180/Tyr182) (#9211), p38 mitogen-activated protein kinase (MAPK) (#9212), p-Akt (Ser473), Akt (#9271), p-mTOR (Ser2448) (#5536), and mTOR antibodies (#2974) were acquired from Cell Signaling Technology Inc. (Beverly, MA, USA), while the β-actin (DKM9001L) antibody was obtained from Sungene Biotech Co., Ltd. (Tianjin, China).

    Techniques: Knockdown, Quantitation Assay, Expressing, Western Blot